Day 5. Grain inoculation with agar - Reishi diary by Shroomok

Prepared sterilized grain bag, agar slant and tools for grain inoculation in Still Air Box. Image from forum thread Day-to-day Reishi growing diary post #5 #3

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• Day-to-day Reishi growing diary

  Day 5. InoculationMy inoculation method is an agar sample.In the photo below, you can see an agar slant with mushroom culture.I mainly use this method for long-term mycelium storage, as well as for multiplying mycelium through agar-to-agar transfers and further inoculation. By the way, agar slants are the best method for preserving genetics for years. From my experience—at least 3 years for sure!If you are interested in this technique, check out the detailed guide here: Agar SlantsSince I had to open the bags for agar inoculation, I proceeded in the Still Air Box.All tools, agar and grain bag inside SAB. I inoculated only one grain bag with Ganoderma Multipileum also called Antler reishi (it produces deer antler-like mushrooms).After inoculation, I placed the bag in a dark closet and left it to incubate at room temperature. During the colonization period, temperatures fluctuated between 70–77°F (21–25°C).

Uploaded on Aug 25, 2025

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