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Luke

What ratio of spawn/substrate did you use? I didn’t get the best flush with my first tub grow, used a 1:3 ratio

Bea

I’m afraid to open my lid. I live in an old house in a cold/damp climate and have not yet had a successful grow because it always gets contaminated despite dousing myself in rubbing alcohol and wiping everything down always when working with the monotub. I’m sure it’s in the air. I’m trying to keep the lid shut and rely on the humidifier, filter disks and the fan to do the work, but pinning hasn’t started and it’s been 13 days. Whats the minimum amount of fanning I can do?

Shroomok avatar Shroomok

@Victor, I'd recommend using any type of whole grain that is safe for consumption (whether for people or animals) and is not treated with insecticides and fungicides. Avoid using grain intended for farm seedlings. Also, ensure the grain is properly stored and free from mold contamination, as some grain contaminants can produce mycotoxins.

Regarding antibiotics, they are mentioned as an optional step, not a requirement. Simply skip them, focus on proper sterilization using a pressure cooker, and work with contam-free mushroom genetics — you'll succeed for sure!

Best of luck!

Shroomok avatar Shroomok

@Steve, temperature and humidity is the same as for any other Cubensis strain.
For colonization period (grain spawn) maintain temperature 73-81F (23-27C).
For FRUITING period maintain temperature 68-77F (20-25C), humidity 90-95%.

Victor

I see that you suggest using nothing but "organic" grains for spawns and substrates yet you are okay with using antibiotics for killing the bad guys. Many people avoid beef because of antibiotics in the meat. Do you not see a yellow/red flag here since mushrooms can concentrate all kinds of substances within the fruiting bodies? Just a little heads up is all. I personally would be very upset if I found out that my lovely mushrooms I was about to eat had residuals of antibiotics in them. DON'T use antibiotics for mushrooms and little else for that matter.

Stephen Martinez

I love these recipes I have used most of them in my 7 years of mushrooms studying. All except the complete nutrition agar and the cornmeal. Going to try the cornmeal today, and a liter with activated charcoal. Need to get a few more ingredients for the complete agar recipe. Thank you so mush for sharing this and for making this kind of information accessible to the public. So much research and information is wasted on corporations and private research sectors.

Shroomok avatar Shroomok

@mrzstrain, what did you use for inoculation? A spore syringe? What is your colonization temperature?

Depending on various factors, the first signs of mycelium may appear within 3–20 days.

1) Spore syringes generally take longer, while liquid cultures or agar inoculations are much faster.
2) Temperature plays a significant role in mycelium development. At 68–73°F (20-23°C) or lower, spore propagation and mycelium growth are noticeably slower. At 75–81°F, growth is much faster.
3) The quality of substrate sterilization and sterility during inoculation — live contaminants can suppress mycelium growth.

Additionally, mycelium may develop inside the jar/bag in areas that aren’t immediately visible to you.

So, it’s too early to panic. Be patient and wait at least 2–3 weeks ;)

Shroomok avatar Shroomok

@Kitty, let's check the progress on day 18, okay?
Feel free to send me a photo of your cake in DM - Reddit, Discord, IG, X or by sending me an email - you can find all links on the main page in footer. It would be much easier to figure out what's wrong

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