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This experiment was shared by member of Shroomok Discord community:

- What's your airation parameters? Aka gas
- Grower: Sterile air to begin with. Built a box with HEPA filter and UVC bulb in it and put aquarium pump in there.
All will be inoculated from the same plate. 3 will be airated. 1-24/7, 1 - 2/7 and 1 - 3/7

- So what's your negative control?
- Grower: 2 will be control one of which has stir bar. And one will be untouched. I had a 6th but lost him in the PC.

- You should do one O2 airated one airated, one Co2 airated and one untouched. I would tell you airation prob won't help but that's not the spirit of science.
- Grower: O2 setup is still coming. Need to find 02 tank. I have cO2 but no O2 atm. Need to also build Seperate boxes for that... Initial experiment was done on a budget.

- Fair enough!
- Grower: Also thinking about it I don't really want to know if O2 works... Because if it does it will be expensive. Unless I make a little eltro O2/hydrogen generator 🤣

- I mean if it works it's cool and good to know but not like a dealbreaker. I.e if it works, good to know. But like also you could just wait a little and you'll get the same result ya know.
- Grower: The impatient gremlin in my head would always know. Now I wait. First thing to notice is bubbles are an awesome agitator. See next photo here


~ 1 day later~

Grower: Woke up to my first failure this morning with the airated LC experiment 😞 turns out the humidity here and the air pump = lots of moisture in the lines which is saturating and blocking the syringe filters In the top of the jars.
I'll need to make a moisture trap inline

Rocco Di Guiseppe

Just harvesting Jack Frost for the first time ever. I didn't get a lot, yet. Im expecting to get some real pulls. I really wanted to learn more about these And look up about it's strength too.

The 2nd-3rd flushes may be better, so just let them grow ;)

You can also try to add casing or pseudo-casing layer — very often it is a game changer!

More details in this section: https://shroomok.com/en/wiki/Making_mushroom_cake,_spawn_to_bulk,_and_casing#Casing

@Ikaruz, very often Psilocybe Cubensis Mexicube strain is confusing with Psilocybe Mexicana, but they are NOT the same. It's different species!

Quick reference: due to scientific classification (Genus -> Species -> Strain) their genus Psilocybe is the same, but the next "word" in naming Cubensis and Mexicana means different species. And the last "word" like Escondido means strain within the species (within Cubensis).

@Lodi you’re very welcome!

It would be great if you could start a thread on the forum, reply to my questions (see below), and add some photos of your monotub and perhaps grain jars at https://shroomok.com/forum so I can check them out and we can discuss your case. I need to SEE it!

Anyway, here are my thoughts / questions for now:

Do you want your cake to fruit and produce Tampanensis fruit bodies, I mean MUSHROOMS themselves? Or just truffles? Or both?

If you’re after truffles you could skip the S2B step and let them develop right in the grain jars. Although, it’s also possible that truffles are forming inside the bulk substrate in your monotub already ;)

To encourage Tampanensis to produce mushrooms (rather than truffles), you need to cover the top of the cake with a casing layer. You mentioned that your tub is almost fully colonized, so go ahead and apply casing! Then provide fruiting conditions right after casing. Casing can help both trigger fruiting and add extra moisture if your cake is (slightly!) dry.

Lodi

Hi ! Thanks for this detail guide. I hope u can give me some directions cause I’m half way through and idk what to do. So I found this guide a little late and after I spawn my grain jars with liquid culture ( Psilocybe tampanesis) I brake and shake and later on s2b to a monotub. Now I realize I put brown rice when it wasn’t the ideal . Idk if this type of sclerotia it’s gonna grow cause it may be too dry I’ve been spraying spring water on this. This tub it’s almost 100% colonized and I was about to put a casing layer. But after than break and shake idk if I should keep doing it. I also notice the amount of FAE in this strain it’s limited for best results cause basically after sb2 I put it straight to fruiting. So at this point idk what to do.

@kb YES, pre-mixed seed starter mixes based on peat moss/ coir/ vermiculite can totally work. I've even used tomato potting soil for P. Cubensis (as a bulk and pseudo-casing) and cacti soil for a casing layer to fruit Panaeolus and P. Tampanensis.

You can use the substrate as-is, or you can bump it up with gypsum and/or hydrated lime to adjust and stabilize pH. Just check the soil pH on the package first. You don’t need acidic soil! Typically you'll find pH 6.8–7.5, it's good for your purposes. 7.5–8.5 is even better.

As you pointed out, substrate should be fungicide-free 👍 And also without added Trichoderma, which is a common natural root system supporter for cannabis plants, but it’s a real killer for mushrooms. Even though, pasteurization neutralizes Trichoderma it’s safer not to have it in your substrate at all.


Thank you for your warm word and wishing you a magnificent harvests!

kb

I've seen JIffyMix seed starting soil recommended as a casing layer.
It appears to be a very clean mix of peat moss/ coir/ vermiculite that does not contain a fungicide.

My question is - could this kind of mix be used for spawn to bulk substrate?
Or maybe with something added?

I enjoy the convenience of a pre-mixed product and would (of course) pasteurize before using.

Thanks!

And BIG Thank You for this site and the accurate and abundant helpful info you share with the shroom community!

@Max in your case you can either:

1) Pour cold water directly INTO THE BAG. Leave the cake to soak for a few hours. Then remove water and keep the cake in the same bag for further fruiting. No need to clean/wash the bag.

OR

2) Remove the cake from the bag and soak it in a bigger bucket or plastic box/basin. After soaking, place it on a plate or wire rack for the next flush

Max

Just reading up on what your saying about the cakes if I have an all in one grow bag do I clean this out and put the cake back in the same all in one grow bag or just leave it on a sterile plate / tub
Thanks in advance

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