Years ago, in Memphis, I planted P cubensis in the flower bed in front of my ground floor apartment for humor. They grew. They were eaten by something. It was slugs. so, remember to use safe slug bait around the outdoor fruiting area. The slug bait solved the problem of slugs.
Also, if growing sclerotia, view the jar at harvest under a fluorescent black light. The sclerotia fluoresces much brighter than the mycelium.
Has anyone experimented with supplementing the grain media with tryptamine HCl and tryptophan? Syrian Rue seed? ON agar media, both P cubensis and mexicana show a slowing of growth when the concentration of tryptamine HCl is 25 mg/100 ml of water. At 25 and 50 mg/100 ml of water, the center part of teh mycelium colony turns bluish. In Rye grain, mycelium colonization is rapid up to 150-250 mg/100 ml of water. 500 mg/100 ml of water in agar greatly retards growth. The center of the colony turns dark blue almost like contamination. I have not had any samples analyzed for potency yet. I sell Tryptamine HCl if anyone is interested.
1) 2 holes –> one for injection port made of butyl rubber plug or silicone sealer (for inoculation), another – for a separate air filter (for gas exchange in the jar and also for steam flow during the sterilization process).
2) 1 hole –> 2-in-1 –> injection port + air filter made of bactericidal breathable tape.
We'll make inoculation through this one hole covered with tape on the Inoculation step here https://shroomok.com/en/wiki/Inoculation_without_glove_box and this breathable tape also will work as an air filter for gas exchange and for steam flow during sterilization.
You can make 2 separate holes if you want; no problem, it won't be a mistake.
@Chad you can dry them in a food dehydrator if you have one. If not – you can use fan, heat fan, oven, heating radiator or just leave them to dry naturally. Here is the guide with all the examples and useful tips https://shroomok.com/en/wiki/How_to_dry_and_store_psilocybe_mushrooms
@hehue let's imagine you grow mushrooms using the same approach, same substrates, and same colonization & fruiting conditions for ALL strains. It doesn't matter whether you're cultivating Golden Teacher or Tidal Wave – genetic quality will play a major role.
To put it simply-roughly:
If you use MSS, multi spore syringe made of spore print, for inoculation – it always generates many random genetics in one space. How is this possible?
Shrooms need only 2 spores (like Male + Female cells) –> they both propagate –> have love (cross) and exchange with genetic information for reproduction = mycelium producing (this is actually called strain) –> then it multiplies and spreads all over the substrate.
BUT! When you use spore syringe, there are billions of spores and they can create massive amount of such couples. They will compete with each other for nutrients and suppress each other. Eventually, a few winners will prevail, but they will spend a lot of resources for fighting.
Therefore, when you see that mycelium and mushrooms on the same cake look different, and the speed of growth is different, and there are areas with no mushrooms at all – it means that mycelium was produced by different couples in same place.
Like GT here https://shroomok.com/en/wiki/File:golden-teacher-first-grow-ever-0c00ad
Or GT here: https://shroomok.com/en/wiki/File:nice-golden-teacher-fruiting-8fe457
However, you NEVER know and can't predict the final outcome from multi spore cultivation – it may be great, may be average, may be poor.
Completely different story is when you use ISOLATED culture, mycelium created by only ONE couple of spores. Culture isolation requires spending plenty of time (from several weeks to several months) of work with agar (selection and isolation by transfer from agar to agar) or cloning on agar.
In such case, mycelium has no competition; it spreads evenly throughout the substrate and forms perfect mushrooms—as beautiful as the ones typically seen in ads photos. Or, for example, as this isolated GT here: https://shroomok.com/en/wiki/File:Bottle-tek-mushrooms-ready-for-harvesting.jpg
The truth is: you will NEVER get that kind of result from multispore genetics, no matter what kind of strain you have ;-)
GT from all the stuff i got (tidal wave and APE) has grown the worst somehow. after month+ i only got one decent grow, 20 little primordia and 3 early fruits.. while APE is already full of tiny mutant balls and tidal wave had 20 shrooms
@GeorgeS. yes, it's possible to save it and make it fruit. Have you checked this write up? https://shroomok.com/en/wiki/Natalensis_grow_bag_fruiting_and_overlay_problems
LED light is fine, but UV light (and direct sunlight) is not good. More about light for mushrooms I explained here: https://shroomok.com/forum/get-feedback-advice-on-mushroom-cultivation/29/all-in-one-bag-issues#post_148
Superb resource and a gift of your time and expertise to all. I htrust all the good karma you have earned is nourishing your soul and your day. Thank you kindly
That's what I have. Im new to this mushroom growing stuff. So is there any hope for this batch.. also i heard somewhere that led lightning and mycilium don't like each other. Is this astatement true ?
Years ago, in Memphis, I planted P cubensis in the flower bed in front of my ground floor apartment for humor. They grew. They were eaten by something. It was slugs. so, remember to use safe slug bait around the outdoor fruiting area. The slug bait solved the problem of slugs.
Also, if growing sclerotia, view the jar at harvest under a fluorescent black light. The sclerotia fluoresces much brighter than the mycelium.
Has anyone experimented with supplementing the grain media with tryptamine HCl and tryptophan? Syrian Rue seed? ON agar media, both P cubensis and mexicana show a slowing of growth when the concentration of tryptamine HCl is 25 mg/100 ml of water. At 25 and 50 mg/100 ml of water, the center part of teh mycelium colony turns bluish. In Rye grain, mycelium colonization is rapid up to 150-250 mg/100 ml of water. 500 mg/100 ml of water in agar greatly retards growth. The center of the colony turns dark blue almost like contamination. I have not had any samples analyzed for potency yet. I sell Tryptamine HCl if anyone is interested.
Long term mycelium storage on agar slants
@daniel, so glad this was useful!
Still Air Box for Mycology
@Dario, there are 2 options:
1) 2 holes –> one for injection port made of butyl rubber plug or silicone sealer (for inoculation), another – for a separate air filter (for gas exchange in the jar and also for steam flow during the sterilization process).
2) 1 hole –> 2-in-1 –> injection port + air filter made of bactericidal breathable tape.
We'll make inoculation through this one hole covered with tape on the Inoculation step here https://shroomok.com/en/wiki/Inoculation_without_glove_box and this breathable tape also will work as an air filter for gas exchange and for steam flow during sterilization.
You can make 2 separate holes if you want; no problem, it won't be a mistake.
Any option will work. The choice is yours!
How to make jars with substrate for mushroom spawn
Why first there are photos and instructions about two holed lids but then you make only one hole?
How to make jars with substrate for mushroom spawn
@Chad you can dry them in a food dehydrator if you have one. If not – you can use fan, heat fan, oven, heating radiator or just leave them to dry naturally. Here is the guide with all the examples and useful tips https://shroomok.com/en/wiki/How_to_dry_and_store_psilocybe_mushrooms
Hillbilly mushrooms - another Bag Tek harvest
How do you dehydrate them while like that??
Hillbilly mushrooms - another Bag Tek harvest
@hehue let's imagine you grow mushrooms using the same approach, same substrates, and same colonization & fruiting conditions for ALL strains. It doesn't matter whether you're cultivating Golden Teacher or Tidal Wave – genetic quality will play a major role.
To put it simply-roughly:
If you use MSS, multi spore syringe made of spore print, for inoculation – it always generates many random genetics in one space. How is this possible?
Shrooms need only 2 spores (like Male + Female cells) –> they both propagate –> have love (cross) and exchange with genetic information for reproduction = mycelium producing (this is actually called strain) –> then it multiplies and spreads all over the substrate.
BUT! When you use spore syringe, there are billions of spores and they can create massive amount of such couples. They will compete with each other for nutrients and suppress each other. Eventually, a few winners will prevail, but they will spend a lot of resources for fighting.
Therefore, when you see that mycelium and mushrooms on the same cake look different, and the speed of growth is different, and there are areas with no mushrooms at all – it means that mycelium was produced by different couples in same place.
Like GT here https://shroomok.com/en/wiki/File:golden-teacher-first-grow-ever-0c00ad
Or GT here: https://shroomok.com/en/wiki/File:nice-golden-teacher-fruiting-8fe457
However, you NEVER know and can't predict the final outcome from multi spore cultivation – it may be great, may be average, may be poor.
Completely different story is when you use ISOLATED culture, mycelium created by only ONE couple of spores. Culture isolation requires spending plenty of time (from several weeks to several months) of work with agar (selection and isolation by transfer from agar to agar) or cloning on agar.
In such case, mycelium has no competition; it spreads evenly throughout the substrate and forms perfect mushrooms—as beautiful as the ones typically seen in ads photos. Or, for example, as this isolated GT here: https://shroomok.com/en/wiki/File:Bottle-tek-mushrooms-ready-for-harvesting.jpg
The truth is: you will NEVER get that kind of result from multispore genetics, no matter what kind of strain you have ;-)
The magic of Golden Teacher mushroom
GT from all the stuff i got (tidal wave and APE) has grown the worst somehow. after month+ i only got one decent grow, 20 little primordia and 3 early fruits.. while APE is already full of tiny mutant balls and tidal wave had 20 shrooms
The magic of Golden Teacher mushroom
cute
APE mushroom no spores
@Scythianman yes, ready!
Mushrooms can be picked as soon as they open their caps, or just before opening
Tidal Wave fruiting
So when they look like that they ready for picking?
Tidal Wave fruiting
You picked them way way, way too soon!
Yeti magic mushrooms
@GeorgeS. yes, it's possible to save it and make it fruit. Have you checked this write up? https://shroomok.com/en/wiki/Natalensis_grow_bag_fruiting_and_overlay_problems
LED light is fine, but UV light (and direct sunlight) is not good. More about light for mushrooms I explained here: https://shroomok.com/forum/get-feedback-advice-on-mushroom-cultivation/29/all-in-one-bag-issues#post_148
Natalensis growing troubleshooting
@JimmyJ, what a kind comment... it definitely nourished my soul today.
Thank you very much! Wish you all the best 🙏
Disposing of magic mushrooms cakes
Superb resource and a gift of your time and expertise to all. I htrust all the good karma you have earned is nourishing your soul and your day. Thank you kindly
Disposing of magic mushrooms cakes
That's what I have. Im new to this mushroom growing stuff. So is there any hope for this batch.. also i heard somewhere that led lightning and mycilium don't like each other. Is this astatement true ?
Natalensis growing troubleshooting
@Isha, ¡De nada! Me alegra ayudar
Tripping: safety guide
Muy útil! Muchas gracias!
Tripping: safety guide
@GaryNelson if it works, why not! But what's even more tricky and of a question is how do you grow mushrooms?! Or do you pick wild?
How to dry and store psilocybe mushrooms
I dry on red brick in a pot at low temps on a camp stove bc I'm a homeless bum. Tricky.
How to dry and store psilocybe mushrooms