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How to make jars with substrate for mushroom spawn

From Shroomok | Let's grow magic mushrooms
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📖
1.How to grow mushrooms at home
2.Stuff to buy for growing
3.Liquid mushroom spore syringe
4.Choose and prepare substrate
5.Cooking the substrate
👉Preparing Jars for spawn
7.Substrate sterilization (before adding mushroom spores)
8.Inoculation without glove box
9.Incubator control
10.Casing of mushroom cakes
11.Cold shock for mushrooms
12.DIY Grow Box. Mushrooms fruiting conditions
13.Start of fruiting, pinhead initiation
14.How to make mushroom spore prints
15.Mushroom harvesting time
16.How to dry and store mushrooms
17.Rehydration of mushroom cakes
18.Second flush of mushroom harvest
19.Third flush of mushroom harvest
20.Fourth flush of mushroom harvest
21.Fifth flush of mushroom harvest
22.Sixth flush of mushroom harvest
23.Disposing of mushrooms cakes
24.Mushroom growing statistics with charts
25.Mushroom growing experiments
26.Mushroom growing conclusions
27.Overlay & Stroma
28.Mushroom aborts
29.Mushroom metabolites
30.Mushrooms contaminants
31.Mushroom candies and mushroom chocolate recipes
32.Mushroom capsules for microdosing
33.Mushroom microdosing
34.Preparing for psychedelic trip
35.How to deal with bad trip
36.Mushroom tea
37.Liquid spore VS Liquid culture
38.Grain to grain transfer

STEP 4. Day 2. How to prepare spawn jars

On the previous step you've already cooked the substrate for magic mushrooms. Now you should prepare jars for it in order to proceed with sterilization.

For this purpose growers use different kind of jars, containers or spawn bags for substrate with inoculation port and breathable air filter.

What is inoculation port and air filter?

Each container, either a polypropylene heat-resistant bag or jar with lid, must be equipped with an inoculation port and a breathing air filter.

Inoculation port (aka injection port) — is an entry point for a syringe needle containing the liquid spore solution or liquid culture to inoculate the sterile substrate. It must be protected agains any competing microorganisms penetration through it. Bactericidal breathable patch, butyl rubber self-healing injection ports (aka bottle stoppers) or heat-resistant rtv silicone sealant is used for this purpose.

Breathable air filter — is an orifice that provides minimal air exchange during the colonization period of the substrate. It must be protected by bactericidal layer to prevent any competing microorganisms from entering through it. Bactericidal breathing patch, Luer lock syringe filters, and synthetic filter disks are used for this purpose.

Mushroom cultivators practice in different ways. Someone makes two holes in the lid: one for injection port and one for filter. Someone makes several ports for inoculation and several filters for air exchange.

In our growing, inoculation port and air filter is one hole. Less holes - less opportunities for contaminants. At the same time, everything is durable, reliable, simple and cheap. Next, I'll show you how to implement it.

Jars for substrate with lids. What are options?

There is a huge variety of materials that can make life easier for growers. Let's take a look at the different options and alternatives.

❇️ Mason Jars or any kind of Glass Jars with plastic or metal lids are often used for such purposes. Mason jars Lids for consist of two parts: a replaceable disposable lid disc with a rubber seal around the rim and a reusable threaded ring.

Mason jar for substrate. Spawn jars. Psilocybin mushrooms cultivation
Mason jar. Lid with injection port (butyl rubber) and air filter (micropore)

Shroomok's Choice on Amazon:

Ball Wide Mouth Mason Jars with Lids & Bands | 16-oz | 2-Pack - $7.99

Ball Wide Mouth Mason Jars with Lids & Bands | 16-oz | 6-Pack - $24.99

Ball Wide Mouth Mason Jars with Lids & Bands | 24 oz | 2-Pack - $14.99

Ball Wide Mouth Mason Jars with Lids & Bands | 32 oz | 2-Pack - $11.35

Ball Wide Mouth Mason Jars with Lids & Bands | 32 oz | 12-Pack - $38.00


❇️ Lid's components. Each lid should be equipped with an inoculation port (butyl rubber injection port) and a breathing filter (patch or micropore nylon laboratory filter).

Butyl rubber self-healing bottle stoppers are a great option for an inoculation port. Can also be used in conjunction with high temperature, heat resistant silicone to fully secure the port. They can be installed in any kind of lids, substrate containers or spawn bags.

Luer-lock syringe filters are a great option for an air filter. They can be installed in any kind of lids or substrate containers. The syringe filters can be substituted with a bactericidal patch.

Inoculation port - butyl rubber injection port, air filter - Luer lock syringe filter
Inoculation port - butyl rubber injection port, air filter - Luer lock syringe filter

Shroomok's Choice on Amazon:

Synthetic Filter Disc for Air filter - 64 pcs. - $6.99

Mushrooms Self Healing Injection Ports - 100 pcs. - $8.94

Syringe Filters PTFE Hydrophobic - 25 pcs. - $12.99

2-in-1: Synthetic Filter Paper Stickers - 64 pcs & Vial Rubber Stopper Injection Ports - 100 pcs - $12.99


❇️ Glass screw jars with heat-resistant plastic or metal lids. You can prepare your own or buy ready-made lids. Inoculation port - butyl rubber injection port. Filter — laboratory funnel filter or substitute with a patch.

Glass spawn jars with heat resistant polypropylene lids
Glass spawn jars with heat-resistant polypropylene lids

Shroomok's Choice on Amazon:

Wide Mouth Mason Jar Plastic Lid + Syringe Filter + Self Healing Injection Ports - 3 pcs - $13.99

3-in-1: Ready to use Mason Jar Lid, Syringe Filter & Self Healing Injection Ports - 6 pcs - $22.99

❇️ Ready-made heat-resistant polypropylene spawn bags with inoculation port and air filter.

Spawn bags for mushrooms cultivation
Spawn bags for mushrooms cultivation
Spawn bag with injection port and air filter
Spawn bag with injection port and air filter

Shroomok's Choice on Amazon:

Middle Mushroom Spawn Bags with Filter -10/20/50 pcs. - $6.59

Small-Medium Mushroom Culture Bag with Air Filter- 10 pcs. - $9.99

Large Mushroom Grow Bags with Gas Filter - 25 pcs. - $24.95

Autoclavable Polypropylene Grow Bags + Injection Port + CO2 Filter - 10 pcs - $28.63


❇️ Make your own Glass Jars or Mason jars as we do.

Glass jars for substrate with metal lids. Spawn jars for psilocybin mushrooms cultivation
Glass jars for substrate with metal lids. Spawn jars for psilocybin mushrooms cultivation


❇️ Heat-resistant polypropylene autoclavable jars.

Heat-resistant polypropylene jars for substrate
Heat-resistant polypropylene jars for substrate. Lids with one hole for injection port and filter covered with bactericidal patch
Polypropylene jars for spawn
Polypropylene jars for spawn
How to identify heat-resistant polypropylene jars for spawn
How to identify heat-resistant polypropylene jars for spawn. PP symbol

Shroomok's Choice on Amazon:

Polypropylene Jars with Lid for Grain Spawn or LC - 12/24/50-Pack 8/16/32 oz. - $8.99

Polypropylene Jars for Grain Spawn & LC - 24 oz. (16 oz, 32 oz available) - 9 Sets - $9.97

Autoclavable Reusable Polypropylene Jars with CO2 Filter in the Lid |17-oz | - 6 pcs - $19.99


❇️ Heat resistant RTV silicone for DIY self-healing injection port.

Red RTV silicone sealant as injection port for spawn jar
Red RTV silicone sealant as injection port for spawn jar
Heat-resistant rtv silicone sealant as injection port
Heat-resistant rtv silicone sealant as injection port

Shroomok's Choice on Amazon:

High Temperature RTV Silicone Gasket Maker and Sealant - Red - 3 oz. - $6.36

What to use is up to you!

Jars is more predictable in comparison with spawn bags. It is harder to accidentally injure growing mycelium in jars. In addition, it's easier to detect contamination and souring. That's why our guide is about jars. Let's figure out how to prepare jars for substrate quick, easy and cheap on your own.

How to prepare jars for substrate?

To make DIY jars and lids for substrate you need:

  1. Jars with lids (volume, types of jars and lids we are going to discuss below).
  2. Screwdriver/nail/awl/drill with a diameter of 5-10 mm.
  3. Bactericidal breathing patch — 1-2 per lid.
  4. Heat-resistant rtv silicone sealant — optional.


What volume for spawn jars is needed

Any jar that fits your pressure cooker (autoclave or multicooker) from 250 ml to 1 liter. We use 500 ml and 700 ml jars. For us these are the optimal volumes. The shape of the jars does not matter.

Jars for substrate
Polypropylene jars with polypropylene lids or glass jars with metal lids

🔴 Note: The shape of the jars is important for PF-tek growing. Use cylindrical or conic jars without narrow neck (neck should be wider than the bottom), so that you can take out the mushroom cake safe and sound or you can open it up with some extreme demonstarted in the video below:

Jars more than 1 liter are not recommended. Why?

  • First, the jar may not fit in the pressure cooker (multicooker).
  • Secondly, a large volume will require more time to colonize the substrate. Accordingly, more risk for souring and contamination, which can take over the substrate faster than the mycelium of psilocybin mushrooms.
  • Third, it is better to share the risks and make more jars of smaller volume. So that, increase the chances of future mushroom survival.
  • With more jars you can experiment and try different methods. Mycelium in each jar will be genetically unique and there is an opportunity to select the best one.


What kind of jars is needed

We use glass screw jars with metal twist-off lids (withstand temperature over +160°C or 320°F) or Mason jars.

Glass screw jars for substrate with metal twist-off lids
Glass screw jars with metal twist-off lids

And heat-resistant polypropylene jars with polypropylene lids (withstand temperature up to +140°C or 284°F).

Heat-resistant polypropylene jars for substrate
Heat-resistant polypropylene jars for substrate


Pros of glass jars:

  • Availability.
  • Easily withstand higher temperatures than polypropylene jars. This point is especially important if your pressure cooker doesn't allow you to set a specific temperature.

Cons:

  • The jar can crack or break during the sterilization process. You can't use such jar and substrate in it for inoculation.
  • It is more difficult to shake the substrate that has covered by mycelium.

Pros of polypropylene jars:

  • Flexible and elastic (it will be easier to shake the substrate with mycelium in such jars)
  • Don't crack or break during sterilization, as it can happen with glass ones.

Cons:

  • Under the influence of temperature changes, the lid can open, then we can't use such substrate for inoculation. Make sure that the lid is tightly closed before and after sterilization.


In fact, we had no problem either with polypropylene (500 ml) or glass (700 ml) jars. The choice is up to you.


Step-by-step spawn jars and lids preparation

1️⃣ Wash all jars and lids. You can use a dish detergent.

Wash all jars for substrate and lids to it. Jars and lids preparation for growing psilocybin mushrooms
Wash all jars for substrate and lids to it


2️⃣ Make only one hole in each lid for the injection port and air filter. Diameter about 5-10 mm. Polypropylene lids can be burned through with a red-hot nail/awl or a screwdriver.

Make inoculation port and filter in the lid. Jars and lids preparation for growing psilocybin mushrooms
Make inoculation port and filter in the lid


For metal lids you can use a drill or punched with a nail/awl or screwdriver.

Make one hole in metal lid for inoculation port and filter
Make one hole in metal lid for inoculation port and filter. Use drill or screwdriver


Diameter of injection port and air filter is 5-10 mm
Diameter of injection port and air filter is 5-10 mm


3️⃣ Tape inoculation port with a breathing bactericidal patch outside (omnipore or micropore tape). Try to stick the patch so that the breathing dots on the patch show you where the center of the inoculation port is. This will make inoculation more convenient.

Inoculation port with filter in the lid. Preparation jars for substrate and lids to it
Inoculation port with filter in the lid

Shroomok's Choice on Amazon:

3M Micropore Tape 1530-1 (2 rolls) 1 x 10 yards - $4.50

PATCH Eco-Friendly Bamboo Bandages Plastic Free, Zero Waste, Natural - 25 pcs. - $6.29

Micropore Surgical Tape - 3m - 2" x 10 YD - 6 rolls - $8.15

Box of Flexible breathable bandages - 50 pcs. - $11.99

🔴 Important: When you make a hole in the metal lid, it is necessary to bend the metal pieces on the back side and seal this place with a breathing bactericidal patch both - inside and outside!

Metal lid with inoculation port and filter (inside view)
Metal lid with inoculation port and filter (inside view)


Metal lid with inoculation port and filter (outside view)
Metal lid with inoculation port and filter (outside view)

It is necessary, because water drops with rust from the lid accumulated there and contamination can easy get in. For polypropylene jars only one breathing bactericidal patch (outside) is needed.


4️⃣ Inoculation port can be made with heat-resistant rtv silicone sealant. This is a great alternative to butyl rubber bottle stoppers.

rtv silicone injection port
rtv silicone injection port


Fill the hole for the inoculation port with heat-resistant silicone sealant (rtv silicone) on both sides.

Injection port with rtv silocone
Fill inoculation port with rtv silocone on both sides


The silicone that hasn't yet solidified should be pressed lightly with a napkin inside and outside of the lid. So that it takes the shape of a tablet or wax seal. Wait for the sealant to completely solidify.

rtv silicone inoculation port
Press gently with a napkin inside and outside

Don't forget to make another separate hole in such lids for air filter and cover it with a bactericidal patch.


Now jars for substrate are ready! You can proceed and move on — STEP 5. Day 2. Substrate sterilization ▶️

Feel free to ask any questions or share your own experience in Shroomok Discord or Shroomok Telegram Chat or in comments to this post ⬇️⬇️⬇️


< Previous
Next >️
📖
1.How to grow mushrooms at home
2.Stuff to buy for growing
3.Liquid mushroom spore syringe
4.Choose and prepare substrate
5.Cooking the substrate
👉Preparing Jars for spawn
7.Substrate sterilization (before adding mushroom spores)
8.Inoculation without glove box
9.Incubator control
10.Casing of mushroom cakes
11.Cold shock for mushrooms
12.DIY Grow Box. Mushrooms fruiting conditions
13.Start of fruiting, pinhead initiation
14.How to make mushroom spore prints
15.Mushroom harvesting time
16.How to dry and store mushrooms
17.Rehydration of mushroom cakes
18.Second flush of mushroom harvest
19.Third flush of mushroom harvest
20.Fourth flush of mushroom harvest
21.Fifth flush of mushroom harvest
22.Sixth flush of mushroom harvest
23.Disposing of mushrooms cakes
24.Mushroom growing statistics with charts
25.Mushroom growing experiments
26.Mushroom growing conclusions
27.Overlay & Stroma
28.Mushroom aborts
29.Mushroom metabolites
30.Mushrooms contaminants
31.Mushroom candies and mushroom chocolate recipes
32.Mushroom capsules for microdosing
33.Mushroom microdosing
34.Preparing for psychedelic trip
35.How to deal with bad trip
36.Mushroom tea
37.Liquid spore VS Liquid culture
38.Grain to grain transfer

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