Home Shroomok - Home Mushrooms Cultivation Consulting

DIY Grow Box for Magic mushrooms. Fruiting conditions

From Shroomok | Let's grow magic mushrooms
< Previous
Next >️
📖
1.How to grow mushrooms at home
2.Stuff to buy for growing
3.Liquid mushroom spore syringe
4.Choose and prepare substrate
5.Cooking the substrate
6.Preparing Jars for spawn
7.Substrate sterilization (before adding mushroom spores)
8.Inoculation without glove box
9.Incubator control
10.Casing of mushroom cakes
11.Cold shock for mushrooms
👉DIY Grow Box. Mushrooms fruiting conditions
13.Start of fruiting, pinhead initiation
14.How to make mushroom spore prints
15.Mushroom harvesting time
16.How to dry and store mushrooms
17.Rehydration of mushroom cakes
18.Second flush of mushroom harvest
19.Third flush of mushroom harvest
20.Fourth flush of mushroom harvest
21.Fifth flush of mushroom harvest
22.Sixth flush of mushroom harvest
23.Disposing of mushrooms cakes
24.Mushroom growing statistics with charts
25.Mushroom growing experiments
26.Mushroom growing conclusions
27.Overlay & Stroma
28.Mushroom aborts
29.Mushroom metabolites
30.Mushrooms contaminants
31.Mushroom candies and mushroom chocolate recipes
32.Mushroom capsules for microdosing
33.Mushroom microdosing
34.Preparing for psychedelic trip
35.How to deal with bad trip
36.Mushroom tea
37.Liquid spore VS Liquid culture
38.Grain to grain transfer

STEP 9. Day 18-26. How to make Growbox. Fruiting conditions

You've successfully passed Casing stage. For now casing layer colonized by mycelium (about 50-60% of the surface).

It's time to prepare growbox for the second phase of growing — fruiting and harvesting.

This means we will stimulate mushroom mycelium to turn from the vegetative phase of mycelium growth to the generative state - pinhead, primordia formation and fruit bodies growth.


Some growers practice "cold shock" before moving cakes to grow box. Another part of growers are vehement opponents of such approach. Check details: What is Cold shock? Pros and Cons of cold shocking in Mushroom Cultivation section.


Growbox Construction and Necessary Equipment for Mushroom Cultivation

Step-by-step growbox preparation

1️⃣ You need plastic container, aquarium or terrarium for making fruiting chamber (aka growbox). The volume should be about 30 liters. You can use plastic boxes of larger (40-80 liters) or smaller sizes (15-20 liters) as well. Bigger volume of the fruiting chamber is better. Because there will be accumulated less carbon dioxide (CO2) concentration during mushrooms growing. Bigger growbox can be ventilated a bit rarely and it's easier to control the microclimate conditions.

Don't use too small containers!

Wash your box thoroughly with any disinfectant detergent.

Container for growbox. Growing psilocybe cubensis
Container for growbox (choose the volume from 15 liters). Growing psilocybin mushrooms

Shroomok's Choice on Amazon:

Modular Stackable Storage Bins - 64 Quart - 2 Pack - $54.93

Homz, Modular Stackable Storage Bins with Blue Latching Handles, 66 Quart, Clear, 2-Pack - $64.00

Modular Stackable Storage Bins - 112 Quart - 2-Pack - $61.29

Buckle Down Storage Box - 132 Quart - 1 Pack - $54.99


2️⃣ Make about 6-8 holes (diameter about 2-3 cm) on the both sides of growing box, at 15-20 cm from the bottom. Cover them with sterile breathing patch. It's necessary for natural air exchange in your growing box. Because during fruiting period mushrooms need fresh air and they produce carbon dioxide, it is denser than air and goes down. Such ventilation holes help with additional natural ventilation during harvesting period.

Grow box preparation for growing magic mushrooms Psilocybe Cubensis
Growbox preparation for growing psilocybin mushrooms Psilocybe Cubensis


3️⃣ Make a separate hole in the grow box to connect the aquarium air pump. We need it for automatic fresh air exchange.

Air pump for growbox
Air pump for FAE (180 L/hour)
Air pump for FAE (150 L/hour)
Air pump for FAE (150 L/hour)

Shroomok's Choice on Amazon:

Quiet Oxygen Pump for 20-100 Gallon - 2 Outlets - $14.99

Air Pump with Dual Outlets - up to 80 Gallon - $16.98

Adjustable Oxygen Pump for 20-100 Gallon - 2 Outlets - $16.99

Powerful Air Pump - for 20-200 Gallon - 2 Outlets - $19.99


Connect air pump hose.

Air pump for ventilation grow box. Growbox preparation for growing magic mushrooms Psilocybe Cubensis.
Air pump for ventilation grow box. Growbox preparation for growing magic mushrooms


Automatic ventilation for growbox. Growbox preparation for growing magic psilocybin mushrooms Psilocybe Cubensis
Automatic ventilation and humidifier for growbox. Growbox preparation for growing psilocybin mushrooms Psilocybe Cubensis


There is a stone at the end of the aquarium air pump hose; it should be dipped in a jar with water. This will filter the incoming air and maintain humidity.

Growbox with air pump for fresh air exchange and additional humidification
Growbox with air pump for fresh air exchange and additional humidification


4️⃣ Some growers place a layer of exclay on the bottom of the grow box (about 2 cm). It ensures high humidity. At first we did so, but in the process we got a musty smell in our grow box. We removed the exclay and cleaned the grow box. That's why we don't recommend to use exclay in the grow box!

For the PF-tek a layer (1-2 cm) of moist (but not wet!) perlite should be placed at the bottom of the grow box.

Pf tek mushroom cakes in growbox
Pf-tek mushroom cakes in growbox



5️⃣ Mix minimum 200-300 ml of chilled boiled water with 10-15 ml of peroxide. Pour the water with peroxide over the walls of the growbox (convenient with a syringe).

6️⃣ Put all cakes in growbox. Some growers recommend to do cold-shock before placing cakes in growbox. Check the What is Cold shock? Pros and Cons of cold shocking in Mushroom Growing

We'd like to mention that we used cold shock only as an experiment that turned out to be successful. However, we don't recommend to use this method for thermophilic psilocybin mushrooms that grow in the tropics and sub-tropics.

Psilocybin mushrooms cakes in growbox. Growbox preparation for cultivation magic mushrooms Psilocybe Cubensis
Psilocybin mushrooms cakes in growbox


Place PF-cakes on foil or any plastic surface. They shouldn't contact directly with moist perlite.

If you have several PF-cakes in your grow box, there should be at least 5 cm between them to let mushrooms grow on the sides.

PF cakes in growbox. Perlite on the bottom
PF cakes in growbox. Perlite on the bottom. 5-10 cm distance between mushroom cakes


7️⃣ Close the grow box tightly with the lid.

8️⃣ Turn on the LED lamp. The lamp light should be weak in the blue range (~435 nm).

LED Lamp for growbox for mushroom cultivation
LED Lamp for growbox

No need to use powerful lamp. Light is needed for pinhead initiation and to set the direction of growth for mushroom bodies. Diode strip or cold white lamp is also suitable for lighting your grow box.

Natural light is suitable as well, but avoid direct sunlight!

LED lamp for growing box
LED lamp in the blue range (~435 nm) for growing box

Shroomok's Choice on Amazon:

RGB Color Changing Light Bulb W/ Remote Control - $9.99

Desk Lamp with Flexible Gooseneck 3 Level Brightness, Touch Control, Portable - $13.99

LED Pool Light, RGB Color Changing and Battery Powered Waterproof with Remote (3 Pack) - $15.89

Desk Gooseneck Magnifier Lamp with Metal Large Clamp, Magnifying Glass with Adjustable Light - $23.98

LED Desk Lamp, 1 USB Charging Port and 2 AC Power Outlet, 1H Timer - $25.68

LED Desk Lamp with Wireless Charger, USB Charging Port, Touch Control, Auto Timer - $26.99

Led Floor Lamp, 4 Color Temperatures Brightness Levels, Adjustable Gooseneck - $33.99


9️⃣ Add thermometer and hygrometer in the grow box to monitor temperature and relative humidity (RH) level.

Thermometr with hygrometr for growbox
Thermometr with hygrometr for growbox

Shroomok's Choice on Amazon:

2-in-1: Simple Digital Thermometer and Hygrometer - $6.95

Bluetooth Digital Hygrometer, Thermometer with Remote App - $11.99

2-in-1: Professional Digital Hygrometer, Thermometer - $17.99


🔟 Fully equipped growbox with mushroom cakes is ready for fruiting and harvesting period!

Here is the 20th day after inoculation.

Growbox eqiupment for growing magic mushrooms Psilocybe Cubensis at home: plastic box, lamp, air pump, thermometer with hygrometer
Eqiupped growbox for growing magic mushrooms Psilocybe Cubensis at home: plastic box, lamp, air pump, thermometer with hygrometer


Fully equipped growbox for growing magic mushrooms Psilocybe Cubensis: grow box itself, light, ventilation, thermometer and hygrometer
Fully equipped growbox for growing fungi Psilocybe Cubensis: grow box itself, lamp, air pump, thermometer and hygrometer


First day in growbox. 20 days after substrate inoculation
First day in growbox. 20 days after substrate inoculation


Growbox for cultivation psilocybin mushrooms Psilocybe Cubensis at home
Waiting for magic mushrooms. Growbox for cultivation psilocybin mushrooms Psilocybe Cubensis at home


Here is the 24th day after inoculation. 2 cakes of strain Brazil. At first glance, they did not seem to be sufficiently covered with mycelium. In fact, such rhizomorphic mycelium (root-like, filamentous) cover is perfect and more productive than tomentose (fluffy, cloudy) mycelium.

Growbox with psilocybin mycelium cakes. Growing Psilocybe Cubensis (Brazil strain)
Growbox with psilocybin mycelium cakes. Growing Psilocybe Cubensis (Brazil strain)


We made another small growbox (volume is 17 liters) for 2 Brazil cakes. Here you can see our 2 growboxes:

Growbox examples for growing magic fungi: Golden Teacher, Thai, Brazil strains
Growbox examples for cultivation magic mushrooms: Golden Teacher, Thai, Brazil strains


Fruiting conditions for psilocybe mushrooms

To initiate fruiting, we need to lower the temperature, increase humidity, give the mushroom a breath of fresh air, and show it soft diffused light in cloudy weather. Under these conditions, the mushroom understands that the dry period is over and the rainy season has arrived. This means that it can already come out of the underground and produce mushrooms on the surface.

The most important thing for the grower in the coming week is to maintain the microclimate in the greenhouse. This is a set of environmental factors. Only a combination of these factors and their parameters provides the ideal environment for the growth of mushrooms. The final yield will largely depend on this period.

Let's briefly consider the necessary conditions for the next 7-12 days.

❇️ Air temperature +22°...+23°C or 72-73°F

After incubation period we need to decrease the temperature by 5-10°C or 8-12°F to initiate the fruiting phase and pinhead formation. The acceptable limits are +21...+25°C or 70-77°F. But at the lower limit the start and growth can be slowed down. At the upper limit there is a risk that mycelium will switch back to the vegetative phase. In addition, you should take into account that the mycelium itself warms up during growth, and the temperature in the substrate itself will be 1-2° higher.

At this stage, it will be enough to continue cultivation at room temperature. Of course, everything depends on the temperature in your room. So, in the harsh winter months you may need a heater (the same one you use for the incubator). And in the summer months, it may be too hot and you can't do without cooling/air conditioning.

Measure the temperature with an ordinary thermometer.

Thermometer for growbox
Thermometer for growbox

Shroomok's Choice on Amazon:

2-in-1: Simple Digital Thermometer and Hygrometer - $6.95

Bluetooth Digital Hygrometer, Thermometer with Remote App - $11.99

2-in-1: Professional Digital Hygrometer, Thermometer - $17.99

❇️ Relative humidity 95-100%

To maintain required humidity spray the walls of the growbox 1-2 times a day with clean water. When doing so, use a spray-bottle. Never pour or spray water directly on the mycelium.

Make sure that casing layer of the mushroom cakes is moist. Casing layer shouldn't be dry!

You can use a terrarium humidifier, it must be built on top. It can be programmed in the intensity and frequency of spraying to maintain a constant humidity of 95-100%. Or you can turn it on when airing your growbox. Use hygrometer to measure the relative humidity level.

Humidifier for growbox
Humidifier for growbox

Shroomok's Choice on Amazon:

Cheap USB-Powered Mini Humidifier 250ml - $6.99

USB Mini Portable Humidifier 350ml - Led Light - $10.99

Portable Humidifying Unit - 0.7 L - Light and Auto Shut-Off - $29.99

Professional Humidifier/fogger with Extension Tube - 4L Large Tank - $37.99

Professional Humidifier Double Extension Tube, 2 in 1 Joint - 2.2 L Tank - $45.99

Professional Humidifier, Fogger for GrowBox - 2 L Tank - $45.99


❇️ Fresh Air Exchange

As you know, mushrooms breathe oxygen and emit carbon dioxide(CO2). After the temperature drops, the fungus will adapt to the new conditions, mycelium growth will slow down and it will emit less carbon dioxide.

But at this stage it will already be necessary to introduce fresh air 2-3 times a day. Just open the growbox for 3-5 minutes and make a few winks with lid over the mushroom cakes. The smaller the growbox and the more cakes, the more often you need to introduce fresh air.

You can use an air pump to ventilate the growbox on a regular basis.

Air pump for gresh air exchange in growbox
Air pump for gresh air exchange in growbox


Automatic ventilation for growbox. Growbox preparation for growing magic psilocybin mushrooms Psilocybe Cubensis
Automatic ventilation for growbox. Growbox preparation for growing psilocybin mushrooms Psilocybe Cubensis

Shroomok's Choice on Amazon:

Quiet Oxygen Pump for 20-100 Gallon - 2 Outlets - $14.99

Air Pump with Dual Outlets - up to 80 Gallon - $16.98

Adjustable Oxygen Pump for 20-100 Gallon - 2 Outlets - $16.99

Powerful Air Pump - for 20-200 Gallon - 2 Outlets - $19.99


❇️ CO2 level is no more than 600-800 ppm

To measure carbon dioxide levels, you can use a CO2 detector. This device is very helpful, but unfortunately, not cheap. To compare, normal CO2 level outdoor is 400-450 ppm. In general, 600-800 ppm it's CO2 level in room, where breath a few people.

CO2 sensor
Digital carbon dioxide detector

Shroomok's Choice on Amazon:

3-in-1: CO2 Detector, Temperature, Humidity - $39.97

3-in-1: Portable CO2 Detector, Temperature, Humidity - LCD Display - $48.99

Air Quality Monitor: CO2, Temperature Humidity - NDIR Channel Sensor, Rechargeable Battery - $71.99

3-in-1: CO2 Meter, Temperature, Humidity; USB Power & Battery - $89.45

Professional 6-in-1: CO2 Detector, Air Pollution, Temperature, Humidity - $89.99

3-in-1: CO2, Temperature, Humidity, Tabletop & Wall Mountable - $129.99

Smart Home - All-in-One - Radon & Air Quality Monitor - $148.76


❇️ Light 12 hours on/off cycle

Turn on the light for 12 hours a day. Natural light is good as well, but only diffused light. Avoid direct sunlight!

RGB Led Lamp for growbox
RGB Led Lamp for growbox (blue range ~435 nm)
LED lamp for growing box
LED lamp for growing box (cold white light)

Shroomok's Choice on Amazon:

LED RGB Color Changing Light Bulb - $9.99

Desk Lamp with Flexible Gooseneck - Portable, Touch Control - $11.89

LED Light, RGB Color Changing, Waterproof - 3 Pack - $14.29

Desk Gooseneck Magnifier Lamp - Metal Large Clamp - Black - $23.98

LED Desk Lamp, 1 USB Charging Port, 2 AC Power Outlet - Black - $25.68

LED Desk Lamp with Wireless Charger - 5 Lighting Modes - White - $26.99

LED Floor Lamp - 4 Color Temperatures, Adjustable Gooseneck - $33.99


❇️ To improve your growing process and make automated growbox you can use different sensors, timers and all sorts of gadgets. It's up to you. The sky is the limit!

Great thing to use is Smart Plug, so that you can connect all your devices with Remote Control that helps to be informed and change growing conditions from anywhere! Most of them are compatible with Alexa, Echo & Google Home.

Shroomok's Choice on Amazon:

Smart Plug, WiFi Socket - 1 or 4 Pack - $9.99

Smart Plug, Smart Home - 2 Pack - $14.69

Mini Smart Plug - 2 Pack - $16.99

Smart WiFi Heavy Duty Outdoor/Indoor Outlet, Timer - 3 Outlets - $19.99

Outdoor/Indoor Smart Plug Smart Home - 2 Sockets - $21.99

Smart Plug Bluetooth - A Certified - 4 Pack - $23.97


It's just a brief overview of the necessary conditions for the next week after moving mushroom cakes in fruiting chamber.

In the next section we are going to figure out in detail:

  • fruiting factors and their combination
  • peculiarities and life cycle of fungi development
  • pinhead initiation conditions
  • fruiting parameters at different stages of life cycle
  • fruiting conditions to increase overall yield
  • what can go wrong: problems during fruiting period with examples
  • contamination during fruiting period with examples

Now move on — STEP 10. Pinhead Initiation. Growing Parameters for Fruiting period ▶️

Feel free to ask any questions or share your own experience in Shroomok Discord or Shroomok Telegram Chat or in comments to this post ⬇️⬇️⬇️


< Previous
Next >️
📖
1.How to grow mushrooms at home
2.Stuff to buy for growing
3.Liquid mushroom spore syringe
4.Choose and prepare substrate
5.Cooking the substrate
6.Preparing Jars for spawn
7.Substrate sterilization (before adding mushroom spores)
8.Inoculation without glove box
9.Incubator control
10.Casing of mushroom cakes
11.Cold shock for mushrooms
👉DIY Grow Box. Mushrooms fruiting conditions
13.Start of fruiting, pinhead initiation
14.How to make mushroom spore prints
15.Mushroom harvesting time
16.How to dry and store mushrooms
17.Rehydration of mushroom cakes
18.Second flush of mushroom harvest
19.Third flush of mushroom harvest
20.Fourth flush of mushroom harvest
21.Fifth flush of mushroom harvest
22.Sixth flush of mushroom harvest
23.Disposing of mushrooms cakes
24.Mushroom growing statistics with charts
25.Mushroom growing experiments
26.Mushroom growing conclusions
27.Overlay & Stroma
28.Mushroom aborts
29.Mushroom metabolites
30.Mushrooms contaminants
31.Mushroom candies and mushroom chocolate recipes
32.Mushroom capsules for microdosing
33.Mushroom microdosing
34.Preparing for psychedelic trip
35.How to deal with bad trip
36.Mushroom tea
37.Liquid spore VS Liquid culture
38.Grain to grain transfer

Join our community