Here I demonstrate the process of agar inoculation with grain spawn sample. Image from forum thread Day-to-day Reishi growing diary post #11 #2
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Day-to-day Reishi growing diary
Day 20 (Part 2). TransfersI set up a Still Air Box (SAB) and placed all the necessary stuff inside.Then I made transfers in SAB one by one:1) Grain Spawn to AgarI prepared no-pour agar cups (5PP heat-resistant cups) and put grain spawn sample in each cup. This was mainly for demonstration—to show one of the ways to save mycelium.Pro Tip: You can also inoculate an agar plate with grain spawn samples from a pre-made grow kit. This way, you can save the genetics for future attempts and save money! 😉2) Grain to Grain Transfer I used the same spawn bag for Grain to Grain transfer, inoculating three more grain bags with Antler Reishi. This method speeds up colonization significantly.Pro Tip: You can also multiply mycelium from pre-made grow kits using Grain to Grain transfers! Detailed process here: Grain to Grain GuideAt this stage, the mycelium is strong enough, reducing the risk of contamination. Colonization also much faster because you're using active mycelium, and each grain becomes a starting point for mycelial growth. If you introduce just 1 tablespoon of spawn, you’ll create at least 100 active points of mycelium growth!3) Spawn to Bulk Finally, I did a Spawn to Bulk transfer (moving the spawn to the fruiting substrate) to prepare three blocks for mushroom fruiting.Pro Tip: One grain spawn bag (about 300 g or 0.7 lbs) is enough to inoculate 20–30 grain or bulk bags. I only needed one tablespoon per bag, but of course I used much more.The day was all about expanding mycelium and preparing for the main (fruiting) stage. Things are looking great, and I’m excited to see how these blocks develop!
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