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Ash

Thanks so much for this article, it's saved me from throwing away a potentially great grow as I was worried about it being contaminate.
I'll be introducing a lot more fresh air exchange now, and hopefully fix the issue.

Shroomok avatar Shroomok

@VK each method has pros & cons. Play with different options to find the best tek for you. Don't forget to share your results, please ;)

Casing isn't nutritious, so sterilization isn't necessary. We use pausterization (steam heat or in the oven for 1-2 hours at 160-180F or even in microwave). But the main point for casing is pH adjusting. You can find details on Step 8. Casing section

Regarding outdoor growing, yes you can bury wasted cakes in the garden in the shade and get shrooms. It is possible under proper climate conditions (warm, high relative humidity), because P. Cubensis are warm-loving tropical/subtropical shrooms. Check the examples I've shared on the final Step 20: Disposing of shroom cakes

Psilocybe Natalensis (African species) great for indoor and outdoor growing for hot season (77-105°F or +25°..40°C).
Psilocybe Azurescens is possible to grow on an outdoor bed in continental climate.
You can cultivate some edible mushrooms outdoor as well ;)

VK

Wonderful guide! Thank you! I am going to experiment with adding some honey when I rehydrate my substrate. I am also thinking of adding some soil as a casing. I figure it does not have to be sterilized because the mycelium is well established.

Question, have you had much luck growing any mushrooms in your garden?

Trippy

Love all you do shroomok! Your community is amazing and you are so generous with your time and knowledge. Glad you persevered and dedicated the time to developing all these resources for us.

Shroomok avatar Shroomok

Oh Lord, I'm neither a real mycologist, nor a real programmer. Maybe, not a real human as well...

I remember your sniffy messages on Reddit. I'm glad that you follow me, but please, don't tell me what (not) to do.

Calm down, toxic contamination monopolist 🙂

Anyway, thank you Mrs. PhD from the internet for a comment regarding Penicillium, 'cause there were 2 not 100% sure cases in Trichoderma article. As I've mentioned above, some content still get corrections due to "work on mistakes".

P.S: I do not sell "mycology supplies", however I would like to

Racheal Currie Ph.D. (@DayTripperonone)

Wondering if you know that some of your Trichoderma Pictures are actually Penicillium mold? You do realize there are other species of green mold, right? You're not a real mycologist, are you? You shouldn't be teaching mycology when your an amateur yourself. You're just here to profit off of people to buy your mycology supplies. Most of your content is misinformation. Sad thumbs Down👎

Shroomok avatar Shroomok

Hey @Flatty!
How thick is your casing layer?
Usually you just need to wait more time for full smooth colonization and further fruiting.
Just patience for now and work on mistakes for the next attempts :)

You can share a photo on Shroomok Discord or Reddit, so I can check the issue ;)

Flatty

Hi guys, I have done a really bad job with a casing layer and put it on too think and a bit lump. Consequently, the colonised bit underneath isn't really grinning through other than a couple of isolated spots - any ideas? Thanks.

Serp

Partially, @PajammaCreature. lol Like Shroomok said, a cold shock on a shiitake block is useful. Tells the mycelium it's time to fruit, essentially. The slap, while ridiculous, was something I found in the literature and it worked once, so I've never deviated slightly lol

PajammaCreature

@Serp, I did have to get a chuckle out of the freezer and slapping the brick with your admission that “the latter almost certainly adds nothing to the process”. I hope you were being funny. 😅😂

Shroomok avatar Shroomok

@Serp, thanks for useful notes 👍
Temperature shock works really great for shiitake!

A detailed study of stress factors can be found in "Effect of Stress on Fruit Body Initiation of Shiitake Mushroom Lentinula edodes" by professor Tjia Wai Mui (The Chinese University of Hong Kong, 2002).

Professor Tjia Wai Mui used different strains of shiitake in his researches (warm-weather, cold-weather and wide-range strains).

He found the stress gene, which was a trigger for fruiting. Thus, for shiitake, heat shock (at +37°C or 99°F for 3 hours) increased the speed of pinning, while cold shock (at +4°C or 39°F for about 12 hours) increased the number of pinheads and overall yield.

He also found that the effectiveness of temperature shock depends on the genetics. The results vary from strain to strain. Temperature shock, whether it is a heat shock or a cold shock, affects the productivity of warm-weather strains and cold-weather strains differently

Serp

Looks good! Couple things, once the block has had time to darken, that primordia will often be referred to as "popcorning". Just slang nomenclature you might encounter in various lit.

Also, you may want to consider leaving the block out during that period before popcorning and before introducing into the FC. Myc is often strong enough in most strains to fight off contam and time to popcorning can be reduced substantially. Ive also put it in a fridge to emulate winter and slapped the brick to emulate a tree falling before introducing into FC. The latter almost certainly adds nothing to the process.

Nice shiitake. They look tasty!

Shroomok avatar Shroomok

Hey @Flatty!
For incubation period we don't need fresh air exchange. Air filter on your jar/bag is more than enough (for gas exchange) and CO2 for this period is necessary for mycelium development!
You're right, 600-800 ppm - for fruiting period, fresh air initiate fruiting

Flatty

Hi there, is there a recommended CO2 level not to exceed for the incubation period? I see 600-800 for fruiting maybe?

Thanks for great website.

Mycelia P

Thank you shroomok for making this beautiful community, so we can all learn from, and appreciate, each other's accomplishments, and failures. I appreciate having a place to be able to hang out and just socialize with people that have the same interest as me.