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Flatty

Hi guys, I have done a really bad job with a casing layer and put it on too think and a bit lump. Consequently, the colonised bit underneath isn't really grinning through other than a couple of isolated spots - any ideas? Thanks.

Serp

Partially, @PajammaCreature. lol Like Shroomok said, a cold shock on a shiitake block is useful. Tells the mycelium it's time to fruit, essentially. The slap, while ridiculous, was something I found in the literature and it worked once, so I've never deviated slightly lol

PajammaCreature

@Serp, I did have to get a chuckle out of the freezer and slapping the brick with your admission that “the latter almost certainly adds nothing to the process”. I hope you were being funny. 😅😂

Shroomok avatar Shroomok

@Serp, thanks for useful notes 👍
Temperature shock works really great for shiitake!

A detailed study of stress factors can be found in "Effect of Stress on Fruit Body Initiation of Shiitake Mushroom Lentinula edodes" by professor Tjia Wai Mui (The Chinese University of Hong Kong, 2002).

Professor Tjia Wai Mui used different strains of shiitake in his researches (warm-weather, cold-weather and wide-range strains).

He found the stress gene, which was a trigger for fruiting. Thus, for shiitake, heat shock (at +37°C or 99°F for 3 hours) increased the speed of pinning, while cold shock (at +4°C or 39°F for about 12 hours) increased the number of pinheads and overall yield.

He also found that the effectiveness of temperature shock depends on the genetics. The results vary from strain to strain. Temperature shock, whether it is a heat shock or a cold shock, affects the productivity of warm-weather strains and cold-weather strains differently

Serp

Looks good! Couple things, once the block has had time to darken, that primordia will often be referred to as "popcorning". Just slang nomenclature you might encounter in various lit.

Also, you may want to consider leaving the block out during that period before popcorning and before introducing into the FC. Myc is often strong enough in most strains to fight off contam and time to popcorning can be reduced substantially. Ive also put it in a fridge to emulate winter and slapped the brick to emulate a tree falling before introducing into FC. The latter almost certainly adds nothing to the process.

Nice shiitake. They look tasty!

Shroomok avatar Shroomok

Hey @Flatty!
For incubation period we don't need fresh air exchange. Air filter on your jar/bag is more than enough (for gas exchange) and CO2 for this period is necessary for mycelium development!
You're right, 600-800 ppm - for fruiting period, fresh air initiate fruiting

Flatty

Hi there, is there a recommended CO2 level not to exceed for the incubation period? I see 600-800 for fruiting maybe?

Thanks for great website.

Mycelia P

Thank you shroomok for making this beautiful community, so we can all learn from, and appreciate, each other's accomplishments, and failures. I appreciate having a place to be able to hang out and just socialize with people that have the same interest as me.

Clark

My pinheads started coming up made it a half an inch to an inch and stop what temperature and what's going on while they're coming up what temperature should I keep it

HotTubFoot

This is what i've been using for months and it works amazingly well. I cannot tell you how much of a game changer it was vs driving myself crazy doing things in a SAB. Tip for using this type of device: turn off all airflow in your workspace and run a HEPA filter in the room for an hour or so before starting your work. I've left agar plates open for an hour in front of this with no contamination. Ive done agar to agar, grain to grain, liquid culture to grain, lc from bottle to syringes, and more with this setup and had zero contamination. If you're starting out, this thing is your magic bullet seriously.

Shroomok avatar Shroomok

@Angie It's an easy and complicated question. Yield depends on many factors. First of all, genetic (multiculture or isolated culture), teks, fruiting conditions etc.

From 5lb of substrate it is possible to get up to 5lb of mushrooms roughly for FULL fruiting cycle. It means for ~5 flushes in total

Each flush never produces the same amount/weight of shrooms

Usually first 3 flushes are the most productive, after that yield decreases. As a result, one flush can produce 50-100g, another one even 2 lb or nothing

Actually, even low succesfull result more than enough for personal use, isn't it?

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