Publications by Shroomok and Mushroom Cultivation Community. Page 13

STEP 6. How to introduce spores or Liquid Culture in sterile substrate. Sterility requirements. Inoculation process without glovebox, SAB or laminar flow hood

STEP 5. Sterilization in Pressure Cooker or Autoclave to eliminate all forms of microorganisms in substrate (molds, fungi, bacteria, spores). Time vs Pressure for sterilization process. Alternative thermal treatment in multicooker, instant pot and oven

STEP 4. How to prepare Spawn Jars and Spawn Bags by your own and save money. Spawn Jars types: autoclavable polypropylene and glass jars. Components for making Inoculation Port and Air Filter. Recommended volume for Spawn Jars and Spawn Bags

STEP 3. Let’s prepare first substrate for magic mushrooms. Recipes and cooking time for different types of grain. Moisture level and useful additives for mushroom spawn

STEP 2. Overall guide about substrate types for mushroom growing. The difference between substrates: grain for spawn, bulk and casing. Choose right grain for spawn. First step of grain substrate preparation