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Cultivation

Liquid culture gallery on Shroomok | Page #4

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4

First liquid culture. I've left it for 2 weeks and these spots formed on top. Are they new mycelium growing or contamination? #2

First liquid culture. I've left it for 2 weeks and these spots formed on top. Are they new mycelium growing or contamination?

Contamination in my LC?

Liquid culture. Contaminated?

LC with aeration

Airated LC experiment. Liquid Culture aeration tek

Albino Penis Envy liquid culture. Good and clear of any contam?

Using stopcocks for LC jar you don’t have to remove your needles. You don’t leave the syringe attached, you close the valve and remove the syringe

Also you could put a marble in the jar prior to PC. It helps break up the mycelium. That was like 2 weeks worth of growth. Started from cultured agar

I also use LME for liquid culture, but I only use 1/2g of LME to 300ml of distilled water

4 month LC stored in fridge

LC should be almost clear. This is about 4 months old

Golden teacher LC. I put way too much LME in this LC when I made it

Is this LC contaminated? I did not stir this for a week

One more example of liquid culture - this is perfect

This is a good liquid culture example

Bad liquid culture happened within 2 days, just showing examples. The cloudyness is bad. Not the amber color

This LC is only 2 weeks old .25 g LME to 300 ml of water

LME liquid culture. Mycelium loves it. 1 month 1 week old

Jar is entirely mycelium. No problem with nutritions at 45 mins sterilization

Take a look at the following publications

Spore syringe Substrate Sterilization Inoculation Colonization Pinning Fruiting Harvest TEKs Agar Spore print Spore swab Experiments Outdoor Microscopy Growing Problems